Domestic league leading scorer Junior Lokosa of Kano Pillars, and FC Crotone’s Simeon Nwankwo join an expected cast of players which will be whittled down to the final 23 in June.
The squad will be led by team captain John Mikel Obi, and has usual suspects Victor Moses, Ahmed Musa and Odion Ighalo.
On the goalkeeping front, coach Gernot Rohr has named four goalkeepers, Ikechukwu Ezenwa, Francis Uzoho, Daniel Akpeyi and Dele AJiboye.
Nigeria training camp is expected to begin on Monday, 21 May in Uyo, ahead of friendlies against Atletico Madrid and Congo DR.
THE FULL SQUAD
Goalkeepers: Ikechukwu Ezenwa (Enyimba FC); Francis Uzoho (Deportivo La Coruna, Spain); Daniel Akpeyi (Chippa United, South Africa); Dele Ajiboye (Plateau United)
Defenders: Abdullahi Shehu (Bursaspor FC, Turkey); Tyronne Ebuehi (Ado Den Haag, The Netherlands); Olaoluwa Aina (Hull City, England); Elderson Echiejile (Cercle Brugge KSV, Belgium); Brian Idowu (Amkar Perm, Russia); Chidozie Awaziem (Nantes FC, France); William Ekong (Bursaspor FC, Turkey); Leon Balogun (FSV Mainz 05, Germany); Kenneth Omeruo (Kasimpasa FC, Turkey); Stephen Eze (Lokomotiv Plovdiv, Bulgaria)
Midfielders: Mikel John Obi (Tianjin Teda, China); Ogenyi Onazi (Trabzonspor FC, Turkey); Wilfred Ndidi (Leicester City, England); Oghenekaro Etebo (UD Las Palmas, Spain); John Ogu (Hapoel Be’er Sheva, Israel); Uche Agbo (Standard Liege, Belgium); Joel Obi (Torino FC, Italy); Mikel Agu (Bursaspor FC, Turkey)
Forwards: Ahmed Musa (CSKA Moscow, Russia); Kelechi Iheanacho (Leicester City, England); Moses Simon (KAA Gent, Belgium); Victor Moses (Chelsea FC, England); Odion Ighalo (Changchun Yatai, China); Alex Iwobi (Arsenal FC, England); Nwankwo Simeon (Crotone FC, Italy); Junior Lokosa (Kano Pillars)